RNAseq data analysis

Description

This workshop will introduce the best practice bioinformatics methods for processing and analyses of RNA-seq data via a series of lectures and computer practicals.This workshop will introduce the best practice bioinformatics methods for processing and analyses of RNA-seq data via a series of online lectures and computer practicals.

Topics covered

• RNAseq Quality control analysis
• Mapping of short reads
• Quantification of gene expression
• Exploratory data analyses
• Differential gene expression
• Gene set enrichment analysis

Pre-requisites

Required for being able to follow the course and complete the computer exercises:

• Basic knowledge in Linux and R
• Your own laptop with the following specs:
  • At least 6 GB of RAM.
  • Operating system Win10, OS X or Linux
  • At least 30 GB of free storage

Desirable:

• Experience working with NGS data analysis or completed the NBIS workshop “Introduction to Bioinformatics using NGS data”
• Experience working in R or completed the NBIS course “R Programming Foundations for Life Scientists”

Level

beginner

Course leaders

Johan Reimegård, Julie Lorent, Nima Rafati

edu.rnaseq@nbis.se